Polymeric immunoglobulin receptor / PIGR | HUABIO HA723953 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Polymeric immunoglobulin receptor / PIGR

catalog :

HA723953

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH17-84

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Polymeric immunoglobulin receptor / PIGR on different lysates with Rabbit anti-Polymeric immunoglobulin receptor / PIGR antibody (HA723953) at 1/5,000 dilution. Lane 1: A431 cell lysate Lane 2: A549 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 83 kDa Observed band size: 70-100 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723953) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Polymeric immunoglobulin receptor / PIGR on different lysates with Rabbit anti-Polymeric immunoglobulin receptor / PIGR antibody (HA723953) at 1/5,000 dilution. Lane 1: Mouse colon tissue lysate Lane 2: Rat stomach tissue lysate Lane 3: Rat colon tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 83 kDa Observed band size: 70-100 kDa Exposure time: 42 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723953) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Polymeric immunoglobulin receptor / PIGR was immunoprecipitated from 0.2 mg A431 cell lysate with HA723953 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723953 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA723953 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA723953 in A431 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801

product information

SKU :

HA723953

Target name :

Polymeric immunoglobulin receptor / PIGR

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human PIGR aa 1-661.

Uniprot id :

P01833>SwissProt: P01833 Human;SwissProt: O70570 Mouse;SwissProt: P15083 Rat

Host :

Rabbit

Clone number :

PSH17-84

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, Mouse colon tissue lysate, Rat stomach tissue lysate, Rat colon tissue lysate.

Molecular wt :

Predicted band size: 83 kDa

Subcellular location :

Cell membrane; Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage