product summary
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company name :
HUABIO
product type :
antibody
product name :
Myc tag
catalog :
HA723950
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH17-81
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (HA723950) at 1/20,000 dilution.
Lane 1: 293T transfected with Myc-tagged empty control cell lysate
Lane 2: 293T transfected with Myc-tagged Claudin18.2 (C-terminal) cell lysate
Lane 3: 293T transfected with Myc-tagged empty control cell lysate
Lane 4: 293T transfected with Myc-tagged ACAT2 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723950) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Myc tag on different lysates with Rabbit anti-Myc tag antibody (HA723950) at 1/5,000 dilution.
Lane 1: 293T transfected with Myc-tagged empty control cell lysate
Lane 2: 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723950) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunocytochemistry analysis of 293T cells labeling Myc tag with Rabbit anti-Myc tag antibody (HA723950) at 1/15,000 dilution.
293T cells, transfected with Myc-tagged empty control (top, negative) / Myc-tagged Claudin18.2 (C-terminal) (middle, positive) / Myc-tagged Histone H3.1 (N-terminal) (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myc tag antibody (HA723950) at 1/15,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Myc tag (HA601081, red) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA723950
Target name :
Myc tag
Species reactivity :
Species independent
Applications :
WB,IF-Cell,IHC-P,FC,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide EQKLISEEDL conjugated to KLH.
Uniprot id :
>
Host :
Rabbit
Clone number :
PSH17-81
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000-1:20,000
;IF-Cell: 1:15,000
;IHC-P: 1:30,000
;FC: 1:5,000
;IP: 1-2μg/sample
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA723950_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded 293T cells transfected with Myc-tagged Histone H3.1 (N-terminal) with Rabbit anti-Myc tag antibody (HA723950) at 1/30,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723950) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA723950_5.jpg
Pic legend5 :
Flow cytometric analysis of 293T cells transfected with Myc-tagged empty control (left) / 293T cells transfected with Myc-tagged ACAT2 (C-terminal) (right) labeling Myc tag.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723950, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA723950_6.jpg
Pic legend6 :
Myc tag was immunoprecipitated from 0.2 mg 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate with HA723950 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723950 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate (input)
Lane 2: HA723950 IP in 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate
Lane 3: Rabbit IgG instead of HA723950 in 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 42 seconds; ECL: K1801
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA723950_7.jpg
Pic legend7 :
Myc tag was immunoprecipitated from 0.2 mg 293T transfected with Myc-tagged ACAT2 (C-terminal) cell lysate with HA723950 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723950 at 1/20,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with Myc-tagged ACAT2 (C-terminal) cell lysate (input)
Lane 2: HA723950 IP in 293T transfected with Myc-tagged ACAT2 (C-terminal) cell lysate
Lane 3: Rabbit IgG instead of HA723950 in 293T transfected with Myc-tagged ACAT2 (C-terminal) cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 2 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!