Rat ICAM1 | HUABIO HA723927B product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Rat ICAM1

catalog :

HA723927B

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

PSH17-52

reactivity :

rat

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723926) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723928) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Interpolated concentrations of native ICAM1 in rat serum samples. Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curve and corrected for sample dilution. Undiluted samples are rat serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM1 concentration was determined to be 62,341 pg/ml in rat serum.

product information

SKU :

HA723927B

Target name :

Rat ICAM1

Species reactivity :

Rat

Applications :

ELISA(Det),ELISA

Conjugate :

Biotin-conjugated

Immunogen :

Recombinant protein within Rat ICAM1 aa 28-493 (HA211063).

Uniprot id :

Q00238>SwissProt: Q00238 Rat

Host :

Rabbit

Clone number :

PSH17-52

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Rat ICAM1 protein (HA211063).

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-51] to Rat ICAM1 antibody (Capture) (HA723926) or Rabbit monoclonal [PSH17-53] to Rat ICAM1 antibody (Capture) (HA723928) and Recombinant Rat ICAM1 protein (HA211063) as the standard. The reference range value is 31.3-4,000 pg/mL. ;ELISA: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723927B_4.png

Pic legend4 :

Interpolated concentrations of native ICAM1 in rat serum samples. Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curve and corrected for sample dilution. Undiluted samples are rat serum 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM1 concentration was determined to be 21,617 pg/ml in rat serum.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723927B_5.png

Pic legend5 :

Interpolated concentrations of spiked ICAM1 in rat cell culture media samples. Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723927B_6.png

Pic legend6 :

Interpolated concentrations of spiked ICAM1 in rat cell culture media samples. Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage