ICAM1 | HUABIO HA723921 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ICAM1

catalog :

HA723921

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH17-38

reactivity :

mouse

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: small intestine Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ICAM1 antibody (HA723921) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723921) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723921) at 1/10,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (30 µg/Lane) Lane 3: Mouse kidney tissue lysate (30 µg/Lane) Predicted band size: 60 kDa Observed band size: 120 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723921) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA723921

Target name :

ICAM1

Species reactivity :

Mouse

Applications :

IHC-Fr,IHC-P,WB,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse ICAM1 aa 1-485.

Uniprot id :

P13597>SwissProt: P13597 Mouse

Host :

Rabbit

Clone number :

PSH17-38

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse lung tissue, RAW264.7 cell lysate, Mouse spleen tissue lysate, Mouse kidney tissue lysate, RAW264.7, mouse spleen cells.

Molecular wt :

Predicted band size: 60 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:1,000 ;IHC-P: 1:2,000 ;WB: 1:10,000 ;IF-Cell: 1:200 ;FC: 1:2,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723921_4.jpg

Pic legend4 :

Immunocytochemistry analysis of RAW264.7 cells labeling ICAM1 with Rabbit anti-ICAM1 antibody (HA723921) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ICAM1 antibody (HA723921) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723921_5.jpg

Pic legend5 :

Flow cytometric analysis of mouse spleen cells labeling ICAM1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723921, 1/2,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723921_6.jpg

Pic legend6 :

ICAM1 was immunoprecipitated from 0.2 mg mouse spleen tissue lysate with HA723921 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723921 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: HA723921 IP in mouse spleen tissue lysate Lane 3: Rabbit IgG instead of HA723921 in mouse spleen tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 59 seconds; ECL: K1801

more info or order :

HUABIO product webpage