Human ACE2 | HUABIO HA723905 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human ACE2

catalog :

HA723905

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH17-18

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human ACE2 matched pair antibodies Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723904) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ACE2 protein (HAg2008) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723905, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native ACE2 in human serum samples and HeLa cell culture supernatant. Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] The concentrations of ACE2 were measured in duplicates, interpolated from the ACE2 standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE2 concentration was determined to be 1,633 pg/ml in human serum and undetectable in HeLa cell culture supernatant.

image 3 :

Interpolated concentrations of spiked ACE2 in human cell culture media samples. Capture: HA723904, Human ACE2 Rabbit mAb [PSH17-17] Detector: HA723905, Human ACE2 Rabbit mAb [PSH17-18] The concentrations of ACE2 were measured in duplicates, interpolated from the ACE2 standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

product information

SKU :

HA723905

Target name :

Human ACE2

Species reactivity :

Human

Applications :

ELISA(Det)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ACE2 aa 18-740 (HAg2008).

Uniprot id :

Q9BYF1>SwissProt: Q9BYF1 Human

Host :

Rabbit

Clone number :

PSH17-18

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human ACE2 protein (HAg2008).

Subcellular location :

Cell membrane, Cytoplasm, Cell projection, cilium, Apical cell membrane; Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-17] to Human ACE2 antibody (Capture) (HA723904) and Recombinant Human ACE2 protein (HAg2008) as the standard. The reference range value is 78.1-20,000 pg/mL.

more info or order :

HUABIO product webpage