product summary
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company name :
HUABIO
product type :
antibody
product name :
Bcl2-L-13
catalog :
HA723899
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH17-12
reactivity :
human, mouse
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Bcl2-L-13 on different lysates with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/50,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: THP-1 cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: COS-1 cell lysate (20 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 85 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723899) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA723899
Target name :
Bcl2-L-13
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IHC-P,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human Bcl2-L-13 aa 1-350.
Uniprot id :
Q9BXK5>SwissProt: Q9BXK5 Human;SwissProt: P59017 Mouse;Entrez Gene: 312682 Rat
Host :
Rabbit
Clone number :
PSH17-12
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
K-562 cell lysate, THP-1 cell lysate, Raji cell lysate, COS-1 cell lysate, human heart tissue, human placenta tissue, mouse heart tissue, mouse liver tissue, mouse placenta tissue, rat heart tissue, rat liver tissue, rat placenta tissue.
Molecular wt :
Predicted band size: 53 kDa
Subcellular location :
Mitochondrion membrane, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:50,000
;IHC-P: 1:200-1:1,000
;IP: 1-2μg/sample
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA723899_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA723899_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA723899_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA723899_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA723899_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/HA723899_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-Bcl2-L-13 antibody (HA723899) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723899) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img10 :
https://storage.huabio.cn/huabio/productImg/HA723899_10.jpg
Pic legend10 :
Bcl2-L-13 was immunoprecipitated from 0.2 mg K-562 cell lysate with HA723899 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723899 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate (input)
Lane 2: HA723899 IP in K-562 cell lysate
Lane 3: Rabbit IgG instead of HA723899 in K-562 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 3 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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