ATPase Inhibitory Factor 1 / IF1 | HUABIO HA723890 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ATPase Inhibitory Factor 1 / IF1

catalog :

HA723890

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH16-94

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ATPase Inhibitory Factor 1 / IF1 on different lysates with Rabbit anti-ATPase Inhibitory Factor 1 / IF1 antibody (HA723890) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: MCF7 cell lysate Lane 4: HepG2 cell lysate Lane 5: HT-29 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723890) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling ATPase Inhibitory Factor 1 / IF1 with Rabbit anti-ATPase Inhibitory Factor 1 / IF1 antibody (HA723890) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATPase Inhibitory Factor 1 / IF1 antibody (HA723890) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ATPase Inhibitory Factor 1 / IF1 antibody (HA723890) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723890) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA723890

Target name :

ATPase Inhibitory Factor 1 / IF1

Species reactivity :

Human,Monkey

Applications :

WB,IF-Cell,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human ATPase Inhibitory Factor 1 aa 1-106.

Uniprot id :

Q9UII2>SwissProt: Q9UII2 Human

Host :

Rabbit

Clone number :

PSH16-94

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, HepG2 cell lysate, HT-29 cell lysate, COS-1 cell lysate, HeLa, human colon carcinoma tissue, human liver tissue, human heart tissue.

Molecular wt :

Predicted band size: 12 kDa

Subcellular location :

Mitochondrion

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:1,000 ;IHC-P: 1:5,000-1:20,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723890_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ATPase Inhibitory Factor 1 / IF1 antibody (HA723890) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723890) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723890_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-ATPase Inhibitory Factor 1 / IF1 antibody (HA723890) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723890) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723890_6.jpg

Pic legend6 :

Flow cytometric analysis of HeLa cells labeling ATPase Inhibitory Factor 1 / IF1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723890, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723890_7.jpg

Pic legend7 :

ATPase Inhibitory Factor 1 / IF1 was immunoprecipitated from 0.2 mg K-562 cell lysate with HA723890 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723890 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: K-562 cell lysate (input) Lane 2: HA723890 IP in K-562 cell lysate Lane 3: Rabbit IgG instead of HA723890 in K-562 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 59 seconds; ECL: K1801

more info or order :

HUABIO product webpage