product summary
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company name :
HUABIO
product type :
antibody
product name :
ICAM1
catalog :
HA723878
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH16-76
reactivity :
mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723878) at 1/10,000 dilution.
Lane 1: C6 cell lysate (20 µg/Lane)
Lane 2: Mouse kidney tissue lysate (30 µg/Lane)
Lane 3: Mouse spleen tissue lysate (30 µg/Lane)
Lane 4: Mouse liver tissue lysate (30 µg/Lane)
Lane 5: Rat spleen tissue lysate (30 µg/Lane)
Lane 6: Rat kidney tissue lysate (30 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 75-100 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723878) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723878) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60-120 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723878) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA723878
Target name :
ICAM1
Species reactivity :
Mouse,Rat
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within rat ICAM1 aa 1-517.
Uniprot id :
P13597>SwissProt: P13597 Mouse;SwissProt: Q00238 Rat
Host :
Rabbit
Clone number :
PSH16-76
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
C6 cell lysate, Mouse kidney tissue lysate, Mouse spleen tissue lysate, Mouse liver tissue lysate, Rat spleen tissue lysate, Rat kidney tissue lysate, RAW264.7 cell lysate, mouse kidney tissue, mouse lung tissue, rat kidney tissue, rat lung tissue, rat liver tissue.
Molecular wt :
Predicted band size: 60 kDa
Subcellular location :
Membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000-1:10,000
;IHC-P: 1:1,000
Advanced Validation :
Cell treatment (CT)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA723878_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA723878_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA723878_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA723878_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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