Human DR3/TNFRSF25 | HUABIO HA723869 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human DR3/TNFRSF25

catalog :

HA723869

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH16-67

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human DR3/TNFRSF25 matched pair antibodies Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723869) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human DR3/TNFRSF25 protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723870, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of Human DR3/TNFRSF25 matched pair antibodies Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723871, Human DR3/TNFRSF25 Rabbit mAb [PSH16-69] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723869) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human DR3/TNFRSF25 protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723871, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Interpolated concentrations of native DR3/TNFRSF25 in TF-1 and HUVEC extract samples based on a 1,000 µg/ml extract load. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] The concentrations of DR3/TNFRSF25 were measured in duplicates, interpolated from the DR3/TNFRSF25 standard curve and corrected for sample dilution. Undiluted samples are TF-1 extract 25% and HUVEC extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DR3/TNFRSF25 concentration was determined to be 1,188 pg/ml in TF-1 extract and undetectable in HUVEC extract.

product information

SKU :

HA723869

Target name :

Human DR3/TNFRSF25

Species reactivity :

Human

Applications :

ELISA(Cap)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human DR3/TNFRSF25 aa 25-199.

Uniprot id :

Q93038>SwissProt: Q93038 Human

Host :

Rabbit

Clone number :

PSH16-67

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human DR3/TNFRSF25 protein.

Subcellular location :

Cell membrane; Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-68] to Human DR3/TNFRSF25 antibody (Detector) (HA723870) or Rabbit monoclonal [PSH16-69] to Human DR3/TNFRSF25 antibody (Detector) (HA723871) and Recombinant Human DR3/TNFRSF25 protein as the standard. The reference range value is 15.6-4,000 pg/mL.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723869_4.png

Pic legend4 :

Interpolated concentrations of native DR3/TNFRSF25 in TF-1 and HUVEC extract samples based on a 1,000 µg/ml extract load. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723871, Human DR3/TNFRSF25 Rabbit mAb [PSH16-69] The concentrations of DR3/TNFRSF25 were measured in duplicates, interpolated from the DR3/TNFRSF25 standard curve and corrected for sample dilution. Undiluted samples are TF-1 extract 25% and HUVEC extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DR3/TNFRSF25 concentration was determined to be 2,154 pg/ml in TF-1 extract and undetectable in HUVEC extract.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723869_5.png

Pic legend5 :

Interpolated concentrations of spiked DR3/TNFRSF25 in human cell culture media samples. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723870, Human DR3/TNFRSF25 Rabbit mAb [PSH16-68] The concentrations of DR3/TNFRSF25 were measured in triplicates, interpolated from the DR3/TNFRSF25 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723869_6.png

Pic legend6 :

Interpolated concentrations of spiked DR3/TNFRSF25 in human cell culture media samples. Capture: HA723869, Human DR3/TNFRSF25 Rabbit mAb [PSH16-67] Detector: HA723871, Human DR3/TNFRSF25 Rabbit mAb [PSH16-69] The concentrations of DR3/TNFRSF25 were measured in triplicates, interpolated from the DR3/TNFRSF25 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).

more info or order :

HUABIO product webpage