Human RANKL | HUABIO HA723861B product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human RANKL

catalog :

HA723861B

quantity :

100μl

price :

409.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

PSH16-59

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human RANKL matched pair antibodies Capture: HA723860, Human RANKL Rabbit mAb [PSH16-58] Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723860) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RANKL protein (HA211170) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723861, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of Human RANKL matched pair antibodies Capture: HA723862, Human RANKL Rabbit mAb [PSH16-60] Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723862) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human RANKL protein (HA211170) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723861, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Interpolated concentrations of spiked TNFSF11 in human cell culture media samples. Capture: HA723860, Human RANKL Rabbit mAb [PSH16-58] Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59] The concentrations of TNFSF11 were measured in duplicates, interpolated from the TNFSF11 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

product information

SKU :

HA723861B

Target name :

Human RANKL

Species reactivity :

Human

Applications :

ELISA(Det),ELISA

Conjugate :

Biotin-conjugated

Immunogen :

Recombinant protein within Human RANKL aa 140-317 (HA211170).

Uniprot id :

O14788>SwissProt: O14788 Human

Host :

Rabbit

Clone number :

PSH16-59

Isotype :

IgG

Size :

100μl

List Price :

409.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human RANKL protein (HA211170).

Subcellular location :

Cell membrane, Cytoplasm, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-58] to Human RANKL antibody (Capture) (HA723860) or Rabbit monoclonal [PSH16-60] to Human RANKL antibody (Capture) (HA723862) and Recombinant Human RANKL protein (HA211170) as the standard. The reference range value is 15.6-2,000 pg/mL. ;ELISA: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723861B_4.png

Pic legend4 :

Interpolated concentrations of spiked TNFSF11 in human cell culture media samples. Capture: HA723862, Human RANKL Rabbit mAb [PSH16-60] Detector: HA723861, Human RANKL Rabbit mAb [PSH16-59] The concentrations of TNFSF11 were measured in duplicates, interpolated from the TNFSF11 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage