TRPV4 | HUABIO HA723855 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TRPV4

catalog :

HA723855

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH16-51

reactivity :

human, mouse

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TRPV4 on different lysates with Rabbit anti-TRPV4 antibody (HA723855) at 1/1,000 dilution. Lane 1: A375 cell lysate Lane 2: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 90 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723855) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IHC-Fr Species: Mouse Site: kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TRPV4 antibody (HA723855) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723855) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA723855

Target name :

TRPV4

Species reactivity :

Human,Mouse

Applications :

WB,IHC-Fr,IHC-P,IP

Conjugate :

Non-conjugated

Uniprot id :

Q9HBA0>SwissProt: Q9HBA0 Human;SwissProt: Q9EPK8 Mouse

Host :

Rabbit

Clone number :

PSH16-51

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A375 cell lysate, mouse kidney tissue.

Molecular wt :

Predicted band size: 98 kDa

Subcellular location :

Cell membrane, Apical cell membrane, Cell junction, adherens junction, Cell projection, cilium.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-Fr: 1:500 ;IHC-P: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723855_4.jpg

Pic legend4 :

TRPV4 was immunoprecipitated from 0.2 mg A375 cell lysate with HA723855 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723855 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A375 cell lysate (input) Lane 2: HA723855 IP in A375 cell lysate Lane 3: Rabbit IgG instead of HA723855 in A375 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 59 seconds; ECL: K1801

more info or order :

HUABIO product webpage