Mouse ICAM1 | HUABIO HA723833B product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Mouse ICAM1

catalog :

HA723833B

quantity :

100μl

price :

409.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

PSH16-35

reactivity :

mouse

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Mouse ICAM1 matched pair antibodies Capture: HA723832, Mouse ICAM1 Rabbit mAb [PSH16-34] Detector: HA723833, Mouse ICAM1 Rabbit mAb [PSH16-35] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723832) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse ICAM1 protein (HA211066) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723833, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of Mouse ICAM1 matched pair antibodies Capture: HA723834, Mouse ICAM1 Rabbit mAb [PSH16-36] Detector: HA723833, Mouse ICAM1 Rabbit mAb [PSH16-35] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723834) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse ICAM1 protein (HA211066) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723833, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Interpolated concentrations of native ICAM-1 in mouse serum samples. Capture: HA723832, Mouse ICAM1 Rabbit mAb [PSH16-34] Detector: HA723833, Mouse ICAM1 Rabbit mAb [PSH16-35] The concentrations of ICAM-1 were measured in duplicates, interpolated from the ICAM-1 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM-1 concentration was determined to be 930.1 ng/ml in mouse serum.

product information

SKU :

HA723833B

Target name :

Mouse ICAM1

Species reactivity :

Mouse

Applications :

ELISA(Det),ELISA

Conjugate :

Biotin-conjugated

Immunogen :

Recombinant protein within Mouse ICAM1 aa 24-485 (HA211066).

Uniprot id :

P13597>SwissProt: P13597 Mouse

Host :

Rabbit

Clone number :

PSH16-35

Isotype :

IgG

Size :

100μl

List Price :

409.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Mouse ICAM1 protein (HA211066).

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-34] to Mouse ICAM1 antibody (Capture) (HA723832) or Rabbit monoclonal [PSH16-36] to Mouse ICAM1 antibody (Capture) (HA723834) and Recombinant Mouse ICAM1 protein (HA211066) as the standard. The reference range value is 31.3-4,000 pg/mL. ;ELISA: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723833B_4.png

Pic legend4 :

Interpolated concentrations of native ICAM-1 in mouse serum samples. Capture: HA723834, Mouse ICAM1 Rabbit mAb [PSH16-36] Detector: HA723833, Mouse ICAM1 Rabbit mAb [PSH16-35] The concentrations of ICAM-1 were measured in duplicates, interpolated from the ICAM-1 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.125%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM-1 concentration was determined to be 1,171 ng/ml in mouse serum.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723833B_5.png

Pic legend5 :

Interpolated concentrations of native ICAM-1 in mouse LPS and mouse IFN-γ stimulated J774A.1 cell culture supernatant. Capture: HA723832, Mouse ICAM1 Rabbit mAb [PSH16-34] Detector: HA723833, Mouse ICAM1 Rabbit mAb [PSH16-35] J774A.1 cells were stimulated with 1 µg/ml LPS and 100 ng/ml mouse IFN-γ or vehicle control and incubated for 72 hours. The concentrations of ICAM-1 measured in duplicate and interpolated from the ICAM-1 standard curve and corrected for sample dilution. Undiluted samples are as follows: stimulated 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM-1 concentration was determined to be 6,529 pg/ml in LPS and mouse IFN-γ stimulated J774A.1 cell culture supernatant.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723833B_6.png

Pic legend6 :

Interpolated concentrations of native ICAM-1 in mouse LPS and mouse IFN-γ stimulated J774A.1 cell culture supernatant. Capture: HA723834, Mouse ICAM1 Rabbit mAb [PSH16-36] Detector: HA723833, Mouse ICAM1 Rabbit mAb [PSH16-35] J774A.1 cells were stimulated with 1 µg/ml LPS and 100 ng/ml mouse IFN-γ or vehicle control and incubated for 72 hours. The concentrations of ICAM-1 measured in duplicate and interpolated from the ICAM-1 standard curve and corrected for sample dilution. Undiluted samples are as follows: stimulated 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM-1 concentration was determined to be 5,372 pg/ml in LPS and mouse IFN-γ stimulated J774A.1 cell culture supernatant.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723833B_7.png

Pic legend7 :

Interpolated concentrations of spiked ICAM-1 in mouse cell culture media samples. Capture: HA723832, Mouse ICAM1 Rabbit mAb [PSH16-34] Detector: HA723833, Mouse ICAM1 Rabbit mAb [PSH16-35] The concentrations of ICAM-1 were measured in duplicates, interpolated from the ICAM-1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA723833B_8.png

Pic legend8 :

Interpolated concentrations of spiked ICAM-1 in mouse cell culture media samples. Capture: HA723834, Mouse ICAM1 Rabbit mAb [PSH16-36] Detector: HA723833, Mouse ICAM1 Rabbit mAb [PSH16-35] The concentrations of ICAM-1 were measured in duplicates, interpolated from the ICAM-1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage