product summary
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company name :
HUABIO
product type :
antibody
product name :
SCNN1G
catalog :
HA723827
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH16-29
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of SCNN1G on different lysates with Rabbit anti-SCNN1G antibody (HA723827) at 1/5,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: A431 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: COS-1 cell lysate (20 µg/Lane) Lane 5: C2C12 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (20 µg/Lane) Lane 8: C6 cell lysate (20 µg/Lane) Lane 9: Mouse kidney tissue lysate (40 µg/Lane)
Lane 10: Mouse lung tissue lysate (40 µg/Lane)
Lane 11: Mouse colon tissue lysate (40 µg/Lane)
Lane 12: Rat kidney tissue lysate (40 µg/Lane)
Lane 13: Rat lung tissue lysate (40 µg/Lane)
Lane 14: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723827) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of 293T cells labeling SCNN1G with Rabbit anti-SCNN1G antibody (HA723827) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCNN1G antibody (HA723827) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Immunocytochemistry analysis of PC-12 cells labeling SCNN1G with Rabbit anti-SCNN1G antibody (HA723827) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SCNN1G antibody (HA723827) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA723827
Target name :
SCNN1G
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human SCNN1G aa 77-541.
Uniprot id :
P51170>SwissProt: P51170 Human;SwissProt: Q9WU39 Mouse;SwissProt: P37091 Rat
Host :
Rabbit
Clone number :
PSH16-29
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
A549 cell lysate, A431 cell lysate, 293T cell lysate, COS-1 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, Mouse colon tissue lysate, Rat kidney tissue lysate, Rat lung tissue lysate, Rat colon tissue lysate, 293T, PC-12.
Molecular wt :
Predicted band size: 74 kDa
Subcellular location :
Apical cell membrane.
Concentration :
1 mg/ml
Recommended dilutions :
WB: 1:5,000
;IF-Cell: 1:100-1:500
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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