CIP2A | HUABIO HA723817 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CIP2A

catalog :

HA723817

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH16-12

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CIP2A on different lysates with Rabbit anti-CIP2A antibody (HA723817) at 1/5,000 dilution. Lane 1: RD cell lysate (20 µg/Lane) Lane 2: MG-63 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (20 µg/Lane) Lane 5: Hela cell lysate (20 µg/Lane) Lane 6: NIH-3T3 cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Predicted band size: 102 kDa Observed band size: 102 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723817) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

CIP2A was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA723817 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723817 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/50,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA723817 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA723817 in Jurkat cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 2 minutes 6 seconds; ECL: K1801

product information

SKU :

HA723817

Target name :

CIP2A

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within PEP240387 220-280

Uniprot id :

Q8TCG1>SwissProt: Q8TCG1 Human;SwissProt: Q8BWY9 Mouse;Entrez Gene: 360711 Rat

Host :

Rabbit

Clone number :

PSH16-12

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

RD cell lysate, MG-63 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, Hela cell lysate, NIH-3T3 cell lysate, C6 cell lysate.

Molecular wt :

Predicted band size: 102 kDa

Subcellular location :

Cytoplasm, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IP: 1:5,000

more info or order :

HUABIO product webpage