Perforin-1 | HUABIO HA723797 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Perforin-1

catalog :

HA723797

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH15-92

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Perforin-1 on different lysates with Rabbit anti-Perforin-1 antibody (HA723797) at 1/20,000 dilution. Lane 1: NIH/3T3 cell lysate (negative) Lane 2: CTLL-2 cell lysate Lane 3: C2C12 cell lysate (negative) Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 75 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723797) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Perforin-1 on different lysates with Rabbit anti-Perforin-1 antibody (HA723797) at 1/10,000 dilution. Lane 1: Ramos cell lysate (negative) Lane 2: NK-92 cell lysate Lane 3: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723797) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Perforin-1 on rat spleen tissue lysates with Rabbit anti-Perforin-1 antibody (HA723797) at 1/5,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 25 seconds ; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723797) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA723797

Target name :

Perforin-1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse Perforin-1 aa 351-555.

Uniprot id :

P14222>SwissProt: P14222 Human;SwissProt: P10820 Mouse;SwissProt: P35763 Rat

Host :

Rabbit

Clone number :

PSH15-92

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

CTLL-2 cell lysate, NK-92 cell lysate, rat spleen lysates, rat spleen tissue, rat lung tissue, CTLL-2.

Molecular wt :

Predicted band size: 61 kDa

Subcellular location :

Cytoplasm,Cell membrane,extracellular region.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:20,000 ;IF-Cell: 1:500 ;IHC-P: 1:200-1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723797_4.jpg

Pic legend4 :

Immunocytochemistry analysis of CTLL-2 (positive) and NIH/3T3 (negative) labeling Perforin-1 with Rabbit anti-Perforin-1 antibody (HA723797) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Perforin-1 antibody (HA723797) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723797_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Prf1 antibody (HA723797) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723797) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723797_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Prf1 antibody (HA723797) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723797) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage