WWOX | HUABIO HA723750 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

WWOX

catalog :

HA723750

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH15-44

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of WWOX on different lysates with Rabbit anti-WWOX antibody (HA723750) at 1/10,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: COS-1 cell lysate (20 µg/Lane) Lane 7: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723750) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

WWOX was immunoprecipitated from 0.2 mg MCF7 cell lysate with HA723750 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723750 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input) Lane 2: HA723750 IP in MCF7 cell lysate Lane 3: Rabbit IgG instead of HA723750 in MCF7 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 seconds; ECL: K1801

product information

SKU :

HA723750

Target name :

WWOX

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human WWOX aa 1-414.

Uniprot id :

Q9NZC7>SwissProt: Q9NZC7 Human;SwissProt: Q91WL8 Mouse;Entrez Gene: 292041 Rat

Host :

Rabbit

Clone number :

PSH15-44

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, Rat brain tissue lysate.

Molecular wt :

Predicted band size: 47 kDa

Subcellular location :

Cytoplasm, Nucleus, Mitochondrion, Golgi apparatus, Lysosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:10,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage