product summary
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company name :
HUABIO
product type :
antibody
product name :
Beta III Tubulin
catalog :
HA723738
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH15-32
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Beta III Tubulin on different lysates with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/2,500 dilution.
Lane 1: SH-SY5Y cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (negative) (15 µg/Lane)
Lane 3: U-87 MG cell lysate (15 µg/Lane)
Lane 4: Neuro-2a cell lysate (15 µg/Lane)
Lane 5: NIH/3T3 cell lysate (negative) (15 µg/Lane)
Lane 6: Mouse brain tissue lysate (20 µg/Lane)
Lane 7: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723738) at 1/2,500 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Application: IF-Tissue
Species: Mouse
Site: cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/400
image 3 :
Application: IF-Tissue
Species: Rat
Site: cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/400
product information
SKU :
HA723738
Target name :
Beta III Tubulin
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Tissue,IF-Cell,FC,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within mouse Beta III Tubulin aa 401-450.
Uniprot id :
Q13509>SwissProt: Q13509 Human;SwissProt: Q9ERD7 Mouse;SwissProt: Q4QRB4 Rat
Host :
Rabbit
Clone number :
PSH15-32
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SH-SY5Y cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse neuron cells, human cerebellum tissue, mouse E14.5 embryo tissue, mouse cerebellum tissue, rat E14.5 embryo tissue, rat cerebellum tissue, SH-SY5Y, Neuro-2a.
Molecular wt :
Predicted band size: 50 kDa
Subcellular location :
Cytoplasm, cytoskeleton, growth cone, lamellipodium, filopodium.
Concentration :
0.1 mg/mL.
Recommended dilutions :
WB: 1:2,500
;IHC-P: 1:2,000-1:4,000
;IF-Tissue: 1:400
;IF-Cell: 1:500
;FC: 1:1,000-1:10,000
;IP: 1-2μg/sample
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA723738_4.jpg
Pic legend4 :
Immunocytochemistry analysis of mouse neuron cells labeling Beta III Tubulin with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA723738_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723738) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA723738_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723738) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA723738_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723738) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA723738_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723738) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/HA723738_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo tissue with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723738) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img10 :
https://storage.huabio.cn/huabio/productImg/HA723738_10.jpg
Pic legend10 :
Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo tissue with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723738) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img11 :
https://storage.huabio.cn/huabio/productImg/HA723738_11.jpg
Pic legend11 :
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723738) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img12 :
https://storage.huabio.cn/huabio/productImg/HA723738_12.jpg
Pic legend12 :
Immunocytochemistry analysis of SH-SY5Y (positive) and HeLa (negative) labeling Beta III Tubulin with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img13 :
https://storage.huabio.cn/huabio/productImg/HA723738_13.jpg
Pic legend13 :
Immunocytochemistry analysis of Neuro-2a cells labeling Beta III Tubulin with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beta III Tubulin antibody (HA723738) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img14 :
https://storage.huabio.cn/huabio/productImg/HA723738_14.jpg
Pic legend14 :
Flow cytometric analysis of HeLa (left, negative) and SH-SY5Y (right, positive) cells labeling Beta III Tubulin.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723738, 1/10,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img15 :
https://storage.huabio.cn/huabio/productImg/HA723738_15.jpg
Pic legend15 :
Flow cytometric analysis of Neuro-2a cells labeling Beta III Tubulin.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723738, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img16 :
https://storage.huabio.cn/huabio/productImg/HA723738_16.jpg
Pic legend16 :
Beta III Tubulin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA723738 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723738 at 1/500 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: mouse brain tissue lysate (input)
Lane 2: HA723738 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA723738 in mouse brain tissue lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 7 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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