Ly6g | HUABIO HA723696 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ly6g

catalog :

HA723696

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH14-95

reactivity :

mouse

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Flow cytometric analysis of mouse bone marrow cells labeling Ly6g. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723696, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 2 :

Flow cytometric analysis of C57 mouse blood cells labeling Ly6g. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723696, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Ly6g antibody (HA723696) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723696) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA723696

Target name :

Ly6g

Species reactivity :

Mouse

Applications :

FC,IHC-P

Conjugate :

Non-conjugated

Uniprot id :

P35461>SwissProt: P35461 Mouse

Host :

Rabbit

Clone number :

PSH14-95

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse bone marrow, C57 mouse blood, mouse spleen tissue, mouse lung tissue.

Molecular wt :

Predicted band size: 14 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

FC: 1:1,000 ;IHC-P: 1:2,000-1:4,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723696_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Ly6g antibody (HA723696) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723696) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage