Mouse IL-3 | HUABIO HA723683 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Mouse IL-3

catalog :

HA723683

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH14-85

reactivity :

mouse

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Mouse IL-3 matched pair antibodies Capture: HA723683, Mouse IL-3 Rabbit mAb [PSH14-85] Detector: HA723684, Mouse IL-3 Rabbit mAb [PSH14-86] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723683) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-3 protein (HA211168) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723684, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of Mouse IL-3 matched pair antibodies Capture: HA723683, Mouse IL-3 Rabbit mAb [PSH14-85] Detector: HA723686, Mouse IL-3 Rabbit mAb [PSH14-87] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723683) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-3 protein (HA211168) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723686, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Interpolated concentrations of native IL-3 in WEHI-3 cultured for 3 days cell culture supernatant. Capture: HA723683, Mouse IL-3 Rabbit mAb [PSH14-85] Detector: HA723684, Mouse IL-3 Rabbit mAb [PSH14-86] The concentrations of IL-3 were measured in triplicates, interpolated from the IL-3 standard curve and corrected for sample dilution. Undiluted samples are WEHI-3 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean IL-3 concentration was determined to be 6,529 pg/ml in WEHI-3 cell culture supernatant.

product information

SKU :

HA723683

Target name :

Mouse IL-3

Species reactivity :

Mouse

Applications :

ELISA(Cap)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse IL-3 aa 27-166 (HA211168).

Uniprot id :

P01586>SwissProt: P01586 Mouse

Host :

Rabbit

Clone number :

PSH14-85

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Mouse IL-3 protein (HA211168).

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-86] to Mouse IL-3 antibody (Detector) (HA723684) or Rabbit monoclonal [PSH14-87] to Mouse IL-3 antibody (Detector) (HA723686) and Recombinant Mouse IL-3 protein (HA211168) as the standard. The reference range value is 7.8-1,000 pg/mL.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723683_4.png

Pic legend4 :

Interpolated concentrations of native IL-3 in WEHI-3 cultured for 3 days cell culture supernatant. Capture: HA723683, Mouse IL-3 Rabbit mAb [PSH14-85] Detector: HA723686, Mouse IL-3 Rabbit mAb [PSH14-87] The concentrations of IL-3 were measured in triplicates, interpolated from the IL-3 standard curve and corrected for sample dilution. Undiluted samples are WEHI-3 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean IL-3 concentration was determined to be 5,145 pg/ml in WEHI-3 cell culture supernatant.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723683_5.png

Pic legend5 :

Interpolated concentrations of spiked IL-3 in mouse cell culture media samples. Capture: HA723683, Mouse IL-3 Rabbit mAb [PSH14-85] Detector: HA723684, Mouse IL-3 Rabbit mAb [PSH14-86] The concentrations of IL-3 were measured in duplicates, interpolated from the IL-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723683_6.png

Pic legend6 :

Interpolated concentrations of spiked IL-3 in mouse cell culture media samples. Capture: HA723683, Mouse IL-3 Rabbit mAb [PSH14-85] Detector: HA723686, Mouse IL-3 Rabbit mAb [PSH14-87] The concentrations of IL-3 were measured in duplicates, interpolated from the IL-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage