Phospho-TAK1 (S412) | HUABIO HA723662 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-TAK1 (S412)

catalog :

HA723662

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH14-64

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-TAK1 (S412) on different lysates with Rabbit anti-Phospho-TAK1 (S412) antibody (HA723662) at 1/5,000 dilution. Lane 1: K-562 cell lysate Lane 2: C2C12 cell lysate Lane 3: C6 cell lysate Lane 4: HeLa cell lysate Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate Lane 8: PC-12 cell lysate Lane 9: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723662) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of HeLa cells untreated (left) / treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-TAK1 (S412). Cells were fixed and permeabilized. Then stained with the primary antibody (HA723662, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

Flow cytometric analysis of NIH/3T3 cells untreated (left) / treated with 100nM Calyculin A for 30 minutes (right) labeling Phospho-TAK1 (S412). Cells were fixed and permeabilized. Then stained with the primary antibody (HA723662, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA723662

Target name :

Phospho-TAK1 (S412)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser412 of mouse TAK1.

Uniprot id :

O43318>SwissProt: O43318 Human;SwissProt: Q62073 Mouse;SwissProt: P0C8E4 Rat

Host :

Rabbit

Clone number :

PSH14-64

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

K-562 cell lysate, C2C12 cell lysate, C6 cell lysate, HeLa cell lysate, HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate.

Molecular wt :

Predicted band size: 67 kDa

Subcellular location :

Cytoplasm, Cell membrane.

Concentration :

0.2 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;FC: 1:2,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage