product summary
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company name :
HUABIO
product type :
antibody
product name :
ALP
catalog :
HA723629
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH14-35
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA723629 image 1
Western blot analysis of ALP on different lysates with Rabbit anti-ALP antibody (HA723629) at 1/5,000 dilution. Lane 1: Saos-2 cell lysate Lane 2: RD cell lysate Lane 3: U-2 OS cell lysate Lane 4: 293T cell lysate Lane 5: C2C12 cell lysate Lane 6: L6 cell lysate Lane 7: Mouse heart tissue lysate Lane 8: Mouse kidney tissue lysate (negative) Lane 9: Rat heart tissue lysate Lane 10: Rat kidney tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 35 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723629) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA723629 image 2
ALP was immunoprecipitated from 0.2 mg Saos-2 cell lysate with HA723629 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723629 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Saos-2 cell lysate (input) Lane 2: HA723629 IP in Saos-2 cell lysate Lane 3: Rabbit IgG instead of HA723629 in Saos-2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 46 seconds; ECL: K1801
product information
SKU :
HA723629
Target name :
ALP
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human ALP aa 1-364.
Uniprot id :
Q53GG5>SwissProt: Q53GG5 Human;SwissProt: O70209 Mouse;SwissProt: Q66HS7 Rat
Host :
Rabbit
Clone number :
PSH14-35
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Saos-2 cell lysate, RD cell lysate, U-2 OS cell lysate, 293T cell lysate, C2C12 cell lysate, L6 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate.
Molecular wt :
Predicted band size: 39 kDa
Subcellular location :
Cytoplasm, myofibril, sarcomere, Z line.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000 ;IP: 1-2μg/sample
Advanced Validation :
Relative expression (RE)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!