Osteopontin | HUABIO HA723601 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Osteopontin

catalog :

HA723601

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH14-11

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded mouse knee joint tissue with Rabbit anti-Osteopontin antibody (HA723601) at 1/9,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723601) at 1/9,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Application: IHC-Fr Species: Mouse Site: kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Rat Site: kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

product information

SKU :

HA723601

Target name :

Osteopontin

Species reactivity :

Mouse,Rat

Applications :

IHC-P,IHC-Fr,WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse Osteopontin aa 1-294.

Uniprot id :

P10923>SwissProt: P10923 Mouse;SwissProt: P08721 Rat

Host :

Rabbit

Clone number :

PSH14-11

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse knee joint tissue, RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for last 3 hours cell lysate, RAW264.7 add 100nM TPA overnight then treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for last 3 hours cell lysate, RAW264.7 cells treated with 100ng/mL LPS for 4 hours, add 1μg/mL BFA for last 3 hours.

Molecular wt :

Predicted band size: 32 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:9,000 ;IHC-Fr: 1:500 ;WB: 1:5,000 ;IF-Cell: 1:2,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723601_4.jpg

Pic legend4 :

Western blot analysis of Osteopontin on different lysates with Rabbit anti-Osteopontin antibody (HA723601) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours, add 1μg/mL BFA for last 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 add 100nM TPA overnight, then treated with 100ng/mL LPS for 4 hours, add 1μg/mL BFA for last 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 32 kDa Observed band size: 60 kDa Exposure time: Lane 1-2: 46 seconds; Lane 3-4: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723601) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723601_5.jpg

Pic legend5 :

Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 100ng/mL LPS for 4 hours, add 1μg/mL BFA for last 3 hours labeling Osteopontin with Rabbit anti-Osteopontin antibody (HA723601) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Osteopontin antibody (HA723601) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage