product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
PRAK / MK5
catalog :
HA723596
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH14-06
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA723596 image 1
Western blot analysis of PRAK / MK5 on different lysates with Rabbit anti-PRAK / MK5 antibody (HA723596) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: Jurkat cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: MEF cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 52/66 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723596) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA723596 image 2
PRAK / MK5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723596 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723596 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723596 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723596 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 30 seconds; ECL: K1802
product information
SKU :
HA723596
Target name :
PRAK / MK5
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human PRAK / MK5 aa 251-473.
Uniprot id :
Q8IW41>SwissProt: Q8IW41 Human;SwissProt: O54992 Mouse;Entrez Gene: 498183 Rat
Host :
Rabbit
Clone number :
PSH14-06
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, A431 cell lysate, A549 cell lysate, MDA-MB-231 cell lysate, Jurkat cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate, PC-12 cell lysate.
Molecular wt :
Predicted band size: 54 kDa
Subcellular location :
Cytoplasm, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000 ;IP: 1-2μg/sample
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!