Phospho-BCKDHA (S293) | HUABIO HA723592 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-BCKDHA (S293)

catalog :

HA723592

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH14-02

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-BCKDHA (S293) on different lysates with Rabbit anti-Phospho-BCKDHA (S293) antibody (HA723592) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HepG2 cell lysate (10 µg/Lane) Lane 3: NIH/3T3 cell lysate (10 µg/Lane) Lane 4: C6 cell lysate (10 µg/Lane) Lane 5: COS-1 cell lysate (10 µg/Lane) Lane 6: Mouse liver tissue lysate (20 µg/Lane) Lane 7: Rat liver tissue lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour (10 µg/Lane) Predicted band size: 50 kDa Observed band size: 48 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723592) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA723592

Target name :

Phospho-BCKDHA (S293)

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser293 of human BCKDHA.

Uniprot id :

P12694>SwissProt: P12694 Human;SwissProt: P50136 Mouse;SwissProt: P11960 Rat

Host :

Rabbit

Clone number :

PSH14-02

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Mitochondrion matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:20,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage