DR3 / LARD | HUABIO HA723583 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DR3 / LARD

catalog :

HA723583

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-90

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of DR3 / LARD on different lysates with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/5,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: THP-1 cell lysate (20 µg/Lane) Lane 4: HDLM-2 cell lysate (20 µg/Lane) Lane 5: U-87 MG cell lysate (20 µg/Lane) Lane 6: HeLa cell lysate (20 µg/Lane) Lane 7: Neuro-2a cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: C6 cell lysate (20 µg/Lane) Lane 10: Mouse spleen tissue lysate (40 µg/Lane) Lane 11: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723583) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of Jurkat cells labeling DR3 / LARD with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DR3 / LARD antibody (HA723583) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA723583

Target name :

DR3 / LARD

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human DR3 / LARD aa 1-199.

Uniprot id :

Q93038>SwissProt: Q93038 Human;Entrez Gene: 85030 Mouse;Entrez Gene: 500592 Rat

Host :

Rabbit

Clone number :

PSH13-90

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, K-562 cell lysate, THP-1 cell lysate, HDLM-2 cell lysate, U-87 MG cell lysate, HeLa cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Jurkat.

Molecular wt :

Predicted band size: 45 kDa

Subcellular location :

Cell membrane; Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100

more info or order :

HUABIO product webpage