product summary
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company name :
HUABIO
product type :
antibody
product name :
Human Lipoprotein A
catalog :
HA723570
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH13-81
reactivity :
human
application :
ELISA
more info or order :
image
image 1 :
Sandwich ELISA analysis of human Lipoprotein A matched pair antibodies
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723570) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Lipoprotein A protein (HA210979) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723571, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
image 2 :
Sandwich ELISA analysis of human Lipoprotein A matched pair antibodies
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723570) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Lipoprotein A protein (HA210979) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723573, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
image 3 :
Interpolated concentrations of native Lipoprotein A in human urine samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human urine 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 5,193 pg/ml in human urine.
product information
SKU :
HA723570
Target name :
Human Lipoprotein A
Species reactivity :
Human
Applications :
ELISA(Cap)
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human LPA aa 20-1,033 (HA210979).
Uniprot id :
P08519>SwissProt: P08519 Human
Host :
Rabbit
Clone number :
PSH13-81
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Recombinant Human Lipoprotein A protein (HA210979).
Subcellular location :
Extracellular region, plasma lipoprotein particle.
Concentration :
1 mg/mL.
Recommended dilutions :
ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-82] to Human Lipoprotein A antibody (Detector) (HA723571) or Rabbit monoclonal [PSH13-83] to Human Lipoprotein A antibody (Detector) (HA723573) and recombinant Human Lipoprotein A protein (HA210979) as the standard. The reference range value is 15.6-2,000 pg/mL.
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA723570_4.jpg
Pic legend4 :
Interpolated concentrations of native Lipoprotein A in human urine samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human urine 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 4,023 pg/ml in human urine.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA723570_5.jpg
Pic legend5 :
Interpolated concentrations of native Lipoprotein A in human serum samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human serum 0.033%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 4.48 µg/ml in human serum.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA723570_6.jpg
Pic legend6 :
Interpolated concentrations of native Lipoprotein A in human serum samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curve and corrected for sample dilution. Undiluted samples are human serum 0.004%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipoprotein A concentration was determined to be 16.01 µg/ml in human serum.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA723570_7.jpg
Pic legend7 :
Interpolated concentrations of spiked Lipoprotein A in human cell culture media samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723571, Human Lipoprotein A Rabbit mAb [PSH13-82]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA723570_8.jpg
Pic legend8 :
Interpolated concentrations of spiked Lipoprotein A in human cell culture media samples.
Capture: HA723570, Human Lipoprotein A Rabbit mAb [PSH13-81]
Detector: HA723573, Human Lipoprotein A Rabbit mAb [PSH13-83]
The concentrations of Lipoprotein A were measured in duplicates, interpolated from the Lipoprotein A standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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