TBC1D1 | HUABIO HA723560 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TBC1D1

catalog :

HA723560

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-71

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TBC1D1 on different lysates with Rabbit anti-TBC1D1 antibody (HA723560) at 1/10,000 dilution. Lane 1: LNCaP cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Predicted band size: 133 kDa Observed band size: 150 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723560) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TBC1D1 antibody (HA723560) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723560) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TBC1D1 antibody (HA723560) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723560) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA723560

Target name :

TBC1D1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human TBC1D1 aa 1-430.

Uniprot id :

Q86TI0>SwissProt: Q86TI0 Human;SwissProt: Q60949 Mouse;Entrez Gene: 360937 Rat

Host :

Rabbit

Clone number :

PSH13-71

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

LNCaP cell lysate, Jurkat cell lysate, C2C12 cell lysate, PC-12 cell lysate, mouse kidney tissue, mouse testis tissue.

Molecular wt :

Predicted band size: 133 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:10,000-1:50,000 ;IHC-P: 1:200 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723560_4.jpg

Pic legend4 :

TBC1D1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA723560 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723560 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA723560 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA723560 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801

more info or order :

HUABIO product webpage