Phospho-PLC-gamma-1 (S1248) | HUABIO HA723551 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-PLC-gamma-1 (S1248)

catalog :

HA723551

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-62

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-PLC-gamma-1 (S1248) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody (HA723551) at 1/2,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate Lane 3: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723551) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Phospho-PLC-gamma-1 (S1248) was immunoprecipitated from 0.2 mg A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate with HA723551 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723551 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate (input) Lane 2: HA723551 IP in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate Lane 3: Rabbit IgG instead of HA723551 in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801

product information

SKU :

HA723551

Target name :

Phospho-PLC-gamma-1 (S1248)

Species reactivity :

Human

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser1248 of human PLC-gamma-1.

Uniprot id :

P19174>SwissProt: P19174 Human

Host :

Rabbit

Clone number :

PSH13-62

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate.

Molecular wt :

Predicted band size: 149 kDa

Subcellular location :

Cell projection, lamellipodium, ruffle.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:10,000 ;IP: 1-2μg/sample

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage