SOX9 | HUABIO HA723548 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SOX9

catalog :

HA723548

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-59

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA723548

Target name :

SOX9

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-Fr,IHC-P,WB,IF-Cell,IP,FC,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse SOX9 aa 1-450.

Uniprot id :

P48436>SwissProt: P48436 Human;SwissProt: Q04887 Mouse;SwissProt: F1LYL9 Rat

Host :

Rabbit

Clone number :

PSH13-59

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human colon tissue, mouse brain tissue, mouse colon tissue, rat brain tissue, rat colon tissue, NIH/3T3 cell lysate, PANC-1 cell lysate, SW480 cell lysate, MDA-MB-231 cell lysate, SW480, NIH/3T3.

Molecular wt :

Predicted band size: 56 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;IHC-P: 1:1,000 ;WB: 1:5,000 ;IF-Cell: 1:2,000-1:5,000 ;IP: 1-2μg/sample ;FC: 1:1,000 ;ChIP: Use 5 μg for 25 μg of chromatin.

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723548_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723548_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723548_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723548_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA723548_8.jpg

Pic legend8 :

Western blot analysis of SOX9 on different lysates with Rabbit anti-SOX9 antibody (HA723548) at 1/5,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: PANC-1 cell lysate Lane 3: SW480 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: MCF7 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 50-70 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723548) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA723548_9.jpg

Pic legend9 :

Immunocytochemistry analysis of SW480 cells (positive) and MCF7 cells (negative) labeling SOX9 with Rabbit anti-SOX9 antibody (HA723548) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX9 antibody (HA723548) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA723548_10.jpg

Pic legend10 :

Immunocytochemistry analysis of NIH/3T3 cells labeling SOX9 with Rabbit anti-SOX9 antibody (HA723548) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX9 antibody (HA723548) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA723548_11.jpg

Pic legend11 :

SOX9 was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with HA723548 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723548 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MDA-MB-231 cell lysate (input) Lane 2: HA723548 IP in MDA-MB-231 cell lysate Lane 3: Rabbit IgG instead of HA723548 in MDA-MB-231 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 46 seconds; ECL: K1801

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA723548_12.jpg

Pic legend12 :

Flow cytometric analysis of MCF7 (left, negative) and SW480 (right, positive) cells labeling SOX9. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723548, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA723548_13.jpg

Pic legend13 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from SW480 cells with SOX9 (HA723548) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage