DCTN1 / p150-glued | HUABIO HA723534 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DCTN1 / p150-glued

catalog :

HA723534

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-45

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of DCTN1 / p150-glued on different lysates with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/15,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 142 kDa Observed band size: 150 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723534) at 1/15,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

product information

SKU :

HA723534

Target name :

DCTN1 / p150-glued

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-Fr,IHC-P,IF-Cell,FC,IF-Tissue,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human DCTN1 aa 1,051-1,278.

Uniprot id :

Q14203>SwissProt: Q14203 Human;SwissProt: O08788 Mouse;SwissProt: P28023 Rat

Host :

Rabbit

Clone number :

PSH13-45

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HEK-293 cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human brain tissue, mouse brain tissue, HEK-293, Neuro-2a, PC-12.

Molecular wt :

Predicted band size: 142 kDa

Subcellular location :

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, spindle, Nucleus envelope, cell cortex.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:15,000-1:50,000 ;IHC-Fr: 1:500 ;IHC-P: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IF-Tissue: 1:200 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723534_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723534) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723534_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723534) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723534_6.jpg

Pic legend6 :

Immunocytochemistry analysis of HEK-293 cells labeling DCTN1 / p150-glued with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723534_7.jpg

Pic legend7 :

Immunocytochemistry analysis of Neuro-2a cells labeling DCTN1 / p150-glued with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA723534_8.jpg

Pic legend8 :

Immunocytochemistry analysis of PC-12 cells labeling DCTN1 / p150-glued with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DCTN1 / p150-glued antibody (HA723534) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA723534_9.jpg

Pic legend9 :

Flow cytometric analysis of HEK-293 cells labeling DCTN1 / p150-glued. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723534, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA723534_10.jpg

Pic legend10 :

Flow cytometric analysis of Neuro-2a cells labeling DCTN1 / p150-glued. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723534, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA723534_11.jpg

Pic legend11 :

Flow cytometric analysis of PC-12 cells labeling DCTN1 / p150-glued. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723534, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA723534_12.jpg

Pic legend12 :

DCTN1 / p150-glued was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723534 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723534 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723534 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723534 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 50 seconds; ECL: K1801

more info or order :

HUABIO product webpage