CD11c | HUABIO HA723512 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD11c

catalog :

HA723512

quantity :

100μl

price :

385.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-25

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD11c on different lysates with Rabbit anti-CD11c antibody (HA723512) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 starved for 2 hours then add 100ng/mL TPA for 48 hours cell lysate Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C2C12 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse colon tissue lysate (40 µg/Lane) Lane 6: Mouse spleen tissue lysate (40 µg/Lane) Lysates/proteins at 20 µg/Lane. Predicted band size: 129 kDa Observed band size: 140 kDa Exposure time: Lane 1-2: 59 seconds; Lane 3-5: 25 seconds; Lane 6: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723512) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Rabbit anti-CD11c antibody (HA723512) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723512) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA723512

Target name :

CD11c

Species reactivity :

Human,Mouse

Applications :

WB,IHC-Fr,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse CD11c aa 616-936 / 1,169.

Uniprot id :

P20702>SwissProt: P20702 Human;SwissProt: Q9QXH4 Mouse

Host :

Rabbit

Clone number :

PSH13-25

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit multiclonal Antibody

Positive control :

THP-1 starved for 2 hours then add 100ng/mL TPA for 48 hours cell lysate, RAW264.7 cell lysate, Mouse colon tissue lysate, Mouse spleen tissue lysate, human Hodgkin's lymphoma tissue, human spleen tissue, mouse spleen tissue.

Molecular wt :

Predicted band size: 129 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-Fr: 1:500 ;IHC-P: 1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723512_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD11c antibody (HA723512) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723512) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723512_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD11c antibody (HA723512) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723512) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage