CD45 | HUABIO HA723506 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD45

catalog :

HA723506

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH13-01

reactivity :

mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD45 on different lysates with Rabbit anti-CD45 antibody (HA723506) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: C2C12 cell lysate (negative) Lane 3: A20 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 145 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723506) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD45 antibody (HA723506) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723506) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-CD45 antibody (HA723506) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723506) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA723506

Target name :

CD45

Species reactivity :

Mouse

Applications :

WB,IHC-P,IHC-Fr,FC,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse CD45 aa 1-566.

Uniprot id :

P06800>SwissProt: P06800 Mouse

Host :

Rabbit

Clone number :

PSH13-01

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

RAW264.7 cell lysate, A20 cell lysate, mouse spleen tissue, mouse stomach tissue, RAW264.7, C57BL/6 mouse spleen cells.

Molecular wt :

Predicted band size: 145 kDa

Subcellular location :

Cell membrane, Membrane raft, Synapse.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:1,000 ;IHC-Fr: 1:500 ;FC: 1:1,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:200

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723506_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723506_5.jpg

Pic legend5 :

Application: IF-Tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/200

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723506_6.jpg

Pic legend6 :

Immunocytochemistry analysis of RAW264.7 (positive) and C2C12 (negative) labeling CD45 with Rabbit anti-CD45 antibody (HA723506) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD45 antibody (HA723506) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723506_7.jpg

Pic legend7 :

Flow cytometric analysis of C57BL/6 mouse spleen cells labeling CD45. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723506, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage