Human CSF-1-R | HUABIO HA723474 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human CSF-1-R

catalog :

HA723474

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH12-66

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723474) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723475, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native CSF1R in THP-1 and Raji cell extract samples based on a 1000 µg/ml extract load. Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] Interpolated concentration of native CSF1R was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CSF1R concentration was determined to be 3144 pg/mL in THP-1 cell extract and undetectable in Raji cell extract.

image 3 :

Interpolated concentrations of spiked CSF1R in cell culture media samples. Capture: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Detector: HA723475, Human CSF-1-R Rabbit mAb [PSH12-67] The concentrations of CSF1R were measured in duplicates, interpolated from the CSF1R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

product information

SKU :

HA723474

Target name :

Human CSF-1-R

Species reactivity :

Human

Applications :

ELISA(Cap),ELISA(Det)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human CSF-1-R aa 20-517 (HA211149).

Uniprot id :

P07333>SwissProt: P07333 Human

Host :

Rabbit

Clone number :

PSH12-66

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human CSF-1-R protein (HA211149).

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-67] to Human CSF-1-R antibody (Detector) (HA723475) and recombinant Human CSF-1-R (HA211149) protein as the standard. The reference range value is 39.1-5000 pg/mL. ;ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-68] to Human CSF-1-R antibody (Capture) (HA723477) and recombinant Human CSF-1-R protein (HA211149) as the standard. The reference range value is 156-5,000 pg/mL.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723474_4.png

Pic legend4 :

Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723477) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723474, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723474_5.png

Pic legend5 :

Interpolated concentrations of native CSF1R in THP-1 and Raji cell extract samples based on a 1000 µg/ml extract load. Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] Interpolated concentration of native CSF1R was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CSF1R concentration was determined to be 3782 pg/mL in THP-1 cell extract and undetectable in Raji cell extract.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723474_6.png

Pic legend6 :

Interpolated concentrations of spiked CSF1R in cell culture media samples. Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66] The concentrations of CSF1R were measured in duplicates, interpolated from the CSF1R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage