Stanniocalcin 2 / STC-2 | HUABIO HA723414 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Stanniocalcin 2 / STC-2

catalog :

HA723414

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH12-01

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Stanniocalcin 2 / STC-2 on different lysates with Rabbit anti-Stanniocalcin 2 / STC-2 antibody (HA723414) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: T-47D cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 36/40 kDa Exposure time: Lane 1-2: 25 seconds; Lane 3: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Stanniocalcin 2 / STC-2 on different lysates with Rabbit anti-Stanniocalcin 2 / STC-2 antibody (HA723414) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM BFA for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 36/40 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Stanniocalcin 2 / STC-2 was immunoprecipitated from 0.2 mg T-47D cell lysate with HA723414 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723414 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: T-47D cell lysate (input) Lane 2: HA723414 IP in T-47D cell lysate Lane 3: Rabbit IgG instead of HA723414 in T-47D cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 minutes; ECL: K1801

product information

SKU :

HA723414

Target name :

Stanniocalcin 2 / STC-2

Species reactivity :

Human

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human STC-2 aa 1-302.

Uniprot id :

O76061>SwissProt: O76061 Human

Host :

Rabbit

Clone number :

PSH12-01

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 cell lysate, T-47D cell lysate, HepG2 cell lysate, HeLa treated with 10μM BFA for 24 hours cell lysate.

Molecular wt :

Predicted band size: 33 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IP: 1-2μg/sample

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage