Human TNF Receptor II | HUABIO HA723404B product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human TNF Receptor II

catalog :

HA723404B

quantity :

100μl

price :

409.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

PSH11-93

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of human TNF-R2 matched pair antibodies Capture: HA723402, Human TNF Receptor II Rabbit mAb [PSH11-92] Detector: HA723403, Human TNF Receptor II Rabbit mAb [PSH11-93] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723402) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TNF Receptor II protein (HA210950) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723404B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of human TNF-R2 matched pair antibodies Capture: HA723405, Human TNF Receptor II Rabbit mAb [PSH11-94] Detector: HA723403, Human TNF Receptor II Rabbit mAb [PSH11-93] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723405) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TNF Receptor II protein (HA210950) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723404B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Interpolated concentrations of native TNF-R2 in U937, SW620 and HeLa cell culture supernatant. Capture: HA723402, Human TNF Receptor II Rabbit mAb [PSH11-92] Detector: HA723403, Human TNF Receptor II Rabbit mAb [PSH11-93] The concentrations of TNF-R2 were measured in duplicates, interpolated from the TNF-R2 standard curve and corrected for sample dilution. Undiluted samples are U937 cell culture supernatant 100%, SW620 cell culture supernatant 100% and HeLa cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF-R2 concentration was determined to be 392.5 pg/ml in U937 cell culture supernatant, 707.2 pg/ml in SW620 cell culture supernatant and undetectable in HeLa cell culture supernatant.

product information

SKU :

HA723404B

Target name :

Human TNF Receptor II

Species reactivity :

Human

Applications :

ELISA(Det),ELISA

Conjugate :

Biotin-conjugated

Immunogen :

Recombinant protein within Human TNF Receptor II aa 23-257.

Uniprot id :

P20333>SwissProt: P20333 Human

Host :

Rabbit

Clone number :

PSH11-93

Isotype :

IgG

Size :

100μl

List Price :

409.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human TNF Receptor II protein (HA210950).

Molecular wt :

Predicted band size: 48 kDa

Subcellular location :

Cell membrane; Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-92] to Human TNF Receptor II antibody (Capture) (HA723402) and Rabbit monoclonal [PSH11-94] to Human TNF Receptor II antibody (Detector) (HA723405) and Recombinant Human TNF Receptor II protein (HA210950) as the standard. The reference range value is 15.6-2,000 pg/ml. ;ELISA: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723404B_4.png

Pic legend4 :

Interpolated concentrations of native TNF-R2 in U937, SW620 and HeLa cell culture supernatant. Capture: HA723405, Human TNF Receptor II Rabbit mAb [PSH11-94] Detector: HA723403, Human TNF Receptor II Rabbit mAb [PSH11-93] The concentrations of TNF-R2 were measured in duplicates, interpolated from the TNF-R2 standard curve and corrected for sample dilution. Undiluted samples are U937 cell culture supernatant 100%, SW620 cell culture supernatant 100% and HeLa cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF-R2 concentration was determined to be 192.5 pg/ml in U937 cell culture supernatant, 569.2 pg/ml in SW620 cell culture supernatant and undetectable in HeLa cell culture supernatant.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723404B_5.png

Pic legend5 :

Interpolated concentrations of native TNF-R2 in human urine samples. Capture: HA723402, Human TNF Receptor II Rabbit mAb [PSH11-92] Detector: HA723403, Human TNF Receptor II Rabbit mAb [PSH11-93] The concentrations of TNF-R2 were measured in duplicates, interpolated from the TNF-R2 standard curve and corrected for sample dilution. Undiluted samples are human urine 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF-R2 concentration was determined to be 11,279 pg/ml in human urine.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723404B_6.png

Pic legend6 :

Interpolated concentrations of native TNF-R2 in human urine samples. Capture: HA723405, Human TNF Receptor II Rabbit mAb [PSH11-94] Detector: HA723403, Human TNF Receptor II Rabbit mAb [PSH11-93] The concentrations of TNF-R2 were measured in duplicates, interpolated from the TNF-R2 standard curve and corrected for sample dilution. Undiluted samples are human urine 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF-R2 concentration was determined to be 8,928 pg/ml in human urine.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723404B_7.png

Pic legend7 :

Interpolated concentrations of spiked TNF-R2 in human cell culture media samples. Capture: HA723402, Human TNF Receptor II Rabbit mAb [PSH11-92] Detector: HA723403, Human TNF Receptor II Rabbit mAb [PSH11-93] The concentrations of TNF-R2 were measured in duplicates, interpolated from the TNF-R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA723404B_8.png

Pic legend8 :

Interpolated concentrations of spiked TNF-R2 in human cell culture media samples. Capture: HA723405, Human TNF Receptor II Rabbit mAb [PSH11-94] Detector: HA723403, Human TNF Receptor II Rabbit mAb [PSH11-93] The concentrations of TNF-R2 were measured in duplicates, interpolated from the TNF-R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage