Human Siglec 7 | HUABIO HA723393 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human Siglec 7

catalog :

HA723393

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH11-87

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of human Siglec 7 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723392) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Siglec 7 protein (HA210942) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723393, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of human Siglec 7 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723393) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Siglec 7 protein (HA210942) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723395, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Interpolated concentrations of native Siglec-7 in U937 and Jurkat extract samples based on a 1,000 µg/ml extract load. Capture: HA723392, Siglec-7 Rabbit mAb [PSH11-86] Detector: HA723393, Siglec-7 Rabbit mAb [PSH11-87] The concentrations of Siglec-7 were measured in duplicates, interpolated from the Siglec-7 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 25% and Jurkat extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Siglec-7 concentration was determined to be 6,385 pg/ml in U937 extract and undetectable in Jurkat extract.

product information

SKU :

HA723393

Target name :

Human Siglec 7

Species reactivity :

Human

Applications :

ELISA(Cap),ELISA(Det)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Siglec 7 aa 19-353.

Uniprot id :

Q9Y286>SwissProt: Q9Y286 Human

Host :

Rabbit

Clone number :

PSH11-87

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human Siglec 7 protein (HA210942).

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-88] to Human Siglec 7 antibody (Detector) (HA723395) and Recombinant Human Siglec 7 protein (HA210942) as the standard. The reference range value is 62.5-8,000 pg/ml. ;ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-86] to Human Siglec 7 antibody (Capture) (HA723392) and Recombinant Human Siglec 7 protein (HA210942) as the standard. The reference range value is 62.5-8,000 pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723393_4.png

Pic legend4 :

Interpolated concentrations of native Siglec-7 in U937 and Jurkat extract samples based on a 1,000 µg/ml extract load. Capture: HA723393, Siglec-7 Rabbit mAb [PSH11-87] Detector: HA723395, Siglec-7 Rabbit mAb [PSH11-88] The concentrations of Siglec-7 were measured in duplicates, interpolated from the Siglec-7 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 25% and Jurkat extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Siglec-7 concentration was determined to be 1,872 pg/ml in U937 extract and undetectable in Jurkat extract.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723393_5.png

Pic legend5 :

Interpolated concentrations of spiked Siglec-7 in human cell culture media samples. Capture: HA723392, Siglec-7 Rabbit mAb [PSH11-86] Detector: HA723393, Siglec-7 Rabbit mAb [PSH11-87] The concentrations of Siglec-7 were measured in duplicates, interpolated from the Siglec-7 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723393_6.png

Pic legend6 :

Interpolated concentrations of spiked Siglec-7 in human cell culture media samples. Capture: HA723393, Siglec-7 Rabbit mAb [PSH11-87] Detector: HA723395, Siglec-7 Rabbit mAb [PSH11-88] The concentrations of Siglec-7 were measured in duplicates, interpolated from the Siglec-7 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage