Human CCL3 / MIP-1 alpha | HUABIO HA723379B product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human CCL3 / MIP-1 alpha

catalog :

HA723379B

quantity :

100μl

price :

409.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

PSH11-77

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of human CCL3 matched pair antibodies Capture: HA723377, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-76] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723377) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723379B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of human CCL3 matched pair antibodies Capture: HA723380, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-78] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723380) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723379B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Interpolated concentrations of native CLL3 in human THP-1 supernatant treated or untreated with PMA and LPS. Capture: HA723377, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-76] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] Interpolated concentration of native CLL3 was measured in duplicate at different sample concentrations and interpolated from the CLL3 standard curves. Undiluted samples are as follows: untreated 100% and treated 12.5%. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CLL3 concentration was determined to be 27 pg/ml in untreated THP-1 cell culture supernatant and 5,007 pg/mL in treated THP-1 supernatant.

product information

SKU :

HA723379B

Target name :

Human CCL3 / MIP-1 alpha

Species reactivity :

Human

Applications :

ELISA(Det)

Conjugate :

Biotin-conjugated

Immunogen :

Recombinant protein within Human CCL3 aa 24-92 (HA210901).

Uniprot id :

P10147>SwissProt: P10147 Human

Host :

Rabbit

Clone number :

PSH11-77

Isotype :

IgG

Size :

100μl

List Price :

409.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human CCL3 / MIP-1 alpha protein (HA210901).

Molecular wt :

Predicted band size: 10 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-76] to Human CCL3 / MIP-1 alpha antibody (Capture) (HA723377) and Rabbit monoclonal [PSH11-78] to Human CCL3 / MIP-1 alpha antibody (Capture) (HA723380) and Recombinant Human CCL3 / MIP-1 alpha protein (HA210901) as the standard. The reference range value is 3.9-1000 pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723379B_4.png

Pic legend4 :

Interpolated concentrations of native CLL3 in human THP-1 supernatant treated or untreated with PMA and LPS. Capture: HA723380, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-78] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] Interpolated concentration of native CLL3 was measured in duplicate at different sample concentrations and interpolated from the CLL3 standard curves. Undiluted samples are as follows: untreated 100% and treated 12.5%. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CLL3 concentration was determined to be 25 pg/ml in untreated THP-1 cell culture supernatant and 5,277 pg/mL in treated THP-1 supernatant.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723379B_5.png

Pic legend5 :

Interpolated concentrations of spiked CLL3 in cell culture media samples. Capture: HA723377, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-76] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] The concentrations of CLL3 were measured in duplicates, interpolated from the CLL3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723379B_6.png

Pic legend6 :

Interpolated concentrations of spiked CLL3 in cell culture media samples. Capture: HA723380, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-78] Detector: HA723378, Human CCL3 / MIP-1 alpha Rabbit mAb [PSH11-77] The concentrations of CLL3 were measured in duplicates, interpolated from the CLL3 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage