PKM2 | HUABIO HA723370 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PKM2

catalog :

HA723370

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH11-74

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PKM2 on different lysates with Rabbit anti-PKM2 antibody (HA723370) at 1/10,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Lane 3: A549 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: C6 cell lysate (15 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (negative) (20 µg/Lane) Lane 7: COS-1 cell lysate (15 µg/Lane) Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723370) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling PKM2 with Rabbit anti-PKM2 antibody (HA723370) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PKM2 antibody (HA723370) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of NIH/3T3 cells labeling PKM2 with Rabbit anti-PKM2 antibody (HA723370) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PKM2 antibody (HA723370) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA723370

Target name :

PKM2

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IF-Cell,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human PKM2 aa 381-430.

Uniprot id :

P14618-1>SwissProt: P14618-1 Human;SwissProt: P52480-1 Mouse;SwissProt: P11980-2 Rat

Host :

Rabbit

Clone number :

PSH11-74

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, HeLa, NIH/3T3, C6, human breast cancer tissue, human colon cancer tissue, human liver tissue, human testis tissue, mouse liver tissue, mouse testis tissue, rat liver tissue, rat testis tissue.

Molecular wt :

Predicted band size: 58 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:10,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723370_4.jpg

Pic legend4 :

Immunocytochemistry analysis of C6 cells labeling PKM2 with Rabbit anti-PKM2 antibody (HA723370) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PKM2 antibody (HA723370) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723370_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723370_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723370_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA723370_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA723370_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA723370_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA723370_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA723370_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue (negative) with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA723370_13.jpg

Pic legend13 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img14 :

https://storage.huabio.cn/huabio/productImg/HA723370_14.jpg

Pic legend14 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img15 :

https://storage.huabio.cn/huabio/productImg/HA723370_15.jpg

Pic legend15 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue (negative) with Rabbit anti-PKM2 antibody (HA723370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img16 :

https://storage.huabio.cn/huabio/productImg/HA723370_16.jpg

Pic legend16 :

Flow cytometric analysis of HeLa cells labeling PKM2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723370, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img17 :

https://storage.huabio.cn/huabio/productImg/HA723370_17.jpg

Pic legend17 :

Flow cytometric analysis of NIH/3T3 cells labeling PKM2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723370, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img18 :

https://storage.huabio.cn/huabio/productImg/HA723370_18.jpg

Pic legend18 :

Flow cytometric analysis of C6 cells labeling PKM2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723370, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img19 :

https://storage.huabio.cn/huabio/productImg/HA723370_19.jpg

Pic legend19 :

Western blot analysis of PKM2 on different lysates with Rabbit anti-PKM2 antibody (HA723370) at 1/2,000 dilution. Lane 1: Human PKM1 recombinant protein (50 ng/Lane) Lane 2: Human PKM2 recombinant protein (50 ng/Lane) Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723370) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img20 :

https://storage.huabio.cn/huabio/productImg/HA723370_20.jpg

Pic legend20 :

PKM2 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723370 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723370 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723370 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723370 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 12 seconds; ECL: K1801

Pic img21 :

https://storage.huabio.cn/huabio/productImg/HA723370_21.jpg

Pic legend21 :

PKM2 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA723370 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723370 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input) Lane 2: HA723370 IP in RAW264.7 cell lysate Lane 3: Rabbit IgG instead of HA723370 in RAW264.7 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 25 seconds; ECL: K1801

more info or order :

HUABIO product webpage