IGF1 Receptor beta | HUABIO HA723368 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IGF1 Receptor beta

catalog :

HA723368

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH11-72

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IGF1 Receptor beta on different lysates with Rabbit anti-IGF1 Receptor beta antibody (HA723368) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: MDA-MB-231 cell lysate (low expression) (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: A549 cell lysate (20 µg/Lane) Lane 7: HeLa cell lysate (20 µg/Lane) Lane 8: HUVEC cell lysate (20 µg/Lane) Lane 9: NIH/3T3 cell lysate (20 µg/Lane) Lane 10: C2C12 cell lysate (20 µg/Lane) Lane 11: C6 cell lysate (20 µg/Lane) Lane 12: Mouse brain tissue lysate (40 µg/Lane) Lane 13: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 155 kDa Observed band size: 95 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723368) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of NIH/3T3 cells labeling IGF1 Receptor beta with Rabbit anti-IGF1 Receptor beta antibody (HA723368) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IGF1 Receptor beta antibody (HA723368) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA723368

Target name :

IGF1 Receptor beta

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human IGF1 Receptor aa 701-935.

Uniprot id :

P08069>SwissProt: P08069 Human;SwissProt: Q60751 Mouse;SwissProt: P24062 Rat

Host :

Rabbit

Clone number :

PSH11-72

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, A431 cell lysate, HepG2 cell lysate, A549 cell lysate, HeLa cell lysate, HUVEC cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, NIH/3T3.

Molecular wt :

Predicted band size: 155 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage