CHD8 | HUABIO HA723367 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CHD8

catalog :

HA723367

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH11-71

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CHD8 on different lysates with Rabbit anti-CHD8 antibody (HA723367) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate (no heat) Lane 3: 293T cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lane 7: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 291 kDa Observed band size: 291 kDa Exposure time: Lane 1-3: 14 seconds; Lane 4-7: 46 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723367) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling CHD8 with Rabbit anti-CHD8 antibody (HA723367) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CHD8 antibody (HA723367) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of NIH/3T3 cells labeling CHD8 with Rabbit anti-CHD8 antibody (HA723367) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CHD8 antibody (HA723367) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA723367

Target name :

CHD8

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,IP,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CHD8 aa 321-570.

Uniprot id :

Q9HCK8>SwissProt: Q9HCK8 Human;SwissProt: Q09XV5 Mouse;SwissProt: Q9JIX5 Rat

Host :

Rabbit

Clone number :

PSH11-71

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, SH-SY5Y cell lysate, 293T cell lysate, NIH/3T3 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HeLa, NIH/3T3, C6, mouse testis tissue, rat testis tissue.

Molecular wt :

Predicted band size: 291 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:100 ;IHC-P: 1:100-1:200 ;IP: 1-2μg/sample ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723367_4.jpg

Pic legend4 :

Immunocytochemistry analysis of C6 cells labeling CHD8 with Rabbit anti-CHD8 antibody (HA723367) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CHD8 antibody (HA723367) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723367_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-CHD8 antibody (HA723367) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723367) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723367_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-CHD8 antibody (HA723367) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723367) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723367_7.jpg

Pic legend7 :

CHD8 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723367 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723367 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723367 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723367 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA723367_8.jpg

Pic legend8 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with CHD8 (HA723367) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage