Human MICA | HUABIO HA723329B product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human MICA

catalog :

HA723329B

quantity :

100μl

price :

409.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

PSH11-40

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of human MICA matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723327) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MICA protein (HA210951) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723329B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native MICA in Hela, K-562 and THP-1 extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native MICA was measured in duplicate at different sample concentrations and interpolated from the MICA standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean MICA concentration was determined to be 7,949 pg/mL in Hela and 1,379 pg/mL in K-562 cell extract. There was no detectable signal in THP-1 cell extract.

image 3 :

Interpolated concentrations of spiked MICA in cell culture media samples. The concentrations of MICA were measured in duplicates, interpolated from the MICA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

product information

SKU :

HA723329B

Target name :

Human MICA

Species reactivity :

Human

Applications :

ELISA(Det),ELISA

Conjugate :

Biotin-conjugated

Immunogen :

Recombinant protein within Human MICA aa 24-308 (HA210951).

Uniprot id :

Q29983>SwissProt: Q29983 Human

Host :

Rabbit

Clone number :

PSH11-40

Isotype :

IgG

Size :

100μl

List Price :

409.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human MICA protein (HA210951).

Subcellular location :

Cell membrane. Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-39] to HumanMICA antibody (Capture) (HA723327) and Recombinant Human Human MICA protein (HA210951) as the standard. The reference range value is 31.2-4,000 pg/mL. ;ELISA: Use at an assay dependent concentration.

more info or order :

HUABIO product webpage