CCL3 / MIP-1 alpha | HUABIO HA723310 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CCL3 / MIP-1 alpha

catalog :

HA723310

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH11-28

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723310) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution. Lane 1: NK-92 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) (20 µg/Lane) Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723310) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours labeling CCL3 / MIP-1 alpha with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA723310

Target name :

CCL3 / MIP-1 alpha

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CCL3 aa 1-92.

Uniprot id :

P10147>SwissProt: P10147 Human;SwissProt: P10855 Mouse

Host :

Rabbit

Clone number :

PSH11-28

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate, RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate, NK-92 cell lysate, RAW264.7 cells treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours, human hodgkin lymphoma tissue, human spleen tissue.

Molecular wt :

Predicted band size: 10 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:2,000 ;IHC-P: 1:200

Advanced Validation :

Cell treatment (CT),Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723310_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human hodgkin lymphoma tissue with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723310_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage