Phospho-MEK3 (S189)+MEK6 (S207) | HUABIO HA723299 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-MEK3 (S189)+MEK6 (S207)

catalog :

HA723299

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH11-17

reactivity :

human, mouse

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-MEK3 (S189)+MEK6 (S207) on different lysates with Rabbit anti-Phospho-MEK3 (S189)+MEK6 (S207) antibody (HA723299) at 1/15,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 39/37 kDa Observed band size: 39/37 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723299) at 1/15,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA723299

Target name :

Phospho-MEK3 (S189)+MEK6 (S207)

Species reactivity :

Human,Mouse

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser189 of Human MEK3 (P46734-2).

Uniprot id :

P46734>SwissProt: P46734 Human;SwissProt: P52564 Human;SwissProt: O09110 Mouse;SwissProt: P70236 Mouse;Entrez Gene: 114495 Rat;Entrez Gene: 303200 Rat

Host :

Rabbit

Clone number :

PSH11-17

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HeLa treated with 25μg/mL Anisomycin for 30 minutes cell lysate, NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate.

Molecular wt :

Predicted band size: 39/37 kDa

Subcellular location :

Cytoplasm, cytosol, membrane, nucleoplasm; Nucleus, Cytoplasm, cytoskeleton.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:15,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage