cIAP1 | HUABIO HA723283B product information

product summary

company name :

HUABIO

product type :

antibody

product name :

cIAP1

catalog :

HA723283B

quantity :

100μl

price :

409.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

PSH06-71

reactivity :

human, mouse, rat

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of human cIAP1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723280) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human cIAP1 protein starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723283B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of human cIAP1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723281) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human cIAP1 protein starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA723283B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Interpolated concentrations of native cIAP1 in THP-1 and TF-1 extract samples based on a 1,000 µg/ml extract load. The concentrations of cIAP1 were measured in duplicates, interpolated from the cIAP1 standard curve and corrected for sample dilution. Undiluted samples are THP-1 extract 25% and TF-1 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean cIAP1 concentration was determined to be 1,712 pg/ml in THP-1 extract and 1,800 pg/ml in TF-1 extract.

product information

SKU :

HA723283B

Target name :

cIAP1

Species reactivity :

Human,Mouse,Rat

Applications :

ELISA(Det),ELISA

Conjugate :

Biotin-conjugated

Immunogen :

Recombinant protein within human cIAP1 aa 201-618.

Uniprot id :

Q13490>SwissProt: Q13490 Human;SwissProt: Q62210 Mouse;Entrez Gene: 60371 Rat

Host :

Rabbit

Clone number :

PSH06-71

Isotype :

IgG

Size :

100μl

List Price :

409.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human cIAP1 protein.

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH06-72] to Human cIAP1 antibody (Capture) (HA723280) or Rabbit monoclonal [PSH02-10] to Human cIAP1 antibody (Capture) (HA723281) and Recombinant Human cIAP1 protein as the standard. The reference range value is 62.5-8,000 pg/ml. ;ELISA: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723283B_4.png

Pic legend4 :

Interpolated concentrations of native cIAP1 in THP-1 and TF-1 extract samples based on a 1,000 µg/mL extract load. The concentrations of cIAP1 were interpolated from the cIAP1 standard curves and corrected for sample dilution. Undiluted samples are THP-1 extract 50% and TF-1 extract 50%. The mean cIAP1 concentration was determined to be 1,450 pg/ml in THP-1 extract and 2,235 pg/ml in TF-1 extract.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723283B_5.png

Pic legend5 :

Interpolated concentrations of spiked cIAP1 in human cell culture media samples. The concentrations of cIAP1 were measured in triplicates, interpolated from the cIAP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723283B_6.png

Pic legend6 :

Interpolated concentrations of spiked cIAP1 in human cell culture media samples. The concentrations of cIAP1 were interpolated from the cIAP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.

more info or order :

HUABIO product webpage