MICA + MICB | HUABIO HA723255 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MICA + MICB

catalog :

HA723255

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-88

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA723255) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (25 µg/Lane) Lane 3: THP-1 cell lysate (negative) (25 µg/Lane) Lane 4: NIH:OVCAR-3 cell lysate (25 µg/Lane) Lane 5: PC-3 cell lysate (low expression) (25 µg/Lane) Lane 6: U-2 OS cell lysate (25 µg/Lane) Lane 7: HUVEC cell lysate (25 µg/Lane) Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723255) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA723255) at 1/2,000 dilution. Lane 1: Human MICA recombinant protein, 20ng/Lane Lane 2: Human MICB recombinant protein, 20ng/Lane Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723255) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Flow cytometric analysis of THP-1 (left, negative) and HeLa (right, positive) cells labeling MICA + MICB. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723255, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA723255

Target name :

MICA + MICB

Species reactivity :

Human

Applications :

WB,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MICB aa 1-309.

Uniprot id :

Q29980>SwissProt: Q29980 Human;SwissProt: Q29983 Human

Host :

Rabbit

Clone number :

PSH10-88

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, MCF7 cell lysate, NIH:OVCAR-3 cell lysate, U-2 OS cell lysate, HUVEC cell lysate, HeLa.

Molecular wt :

Predicted band size: 43 kDa

Subcellular location :

Cell membrane, Cytoplasm; Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723255_4.jpg

Pic legend4 :

MICA + MICB was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723255 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723255 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA723255 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA723255 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801

more info or order :

HUABIO product webpage