DFNA5 / GSDME | HUABIO HA723251 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DFNA5 / GSDME

catalog :

HA723251

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-84

reactivity :

human, mouse

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723251) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/30 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723251) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723251) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mouse GSDME (N-terminal) cell lysate Lane 3: 293T transfected with mouse GSDME (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723251) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA723251 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723251 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input) Lane 2: HA723251 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: Rabbit IgG instead of HA723251 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801

product information

SKU :

HA723251

Target name :

DFNA5 / GSDME

Species reactivity :

Human,Mouse

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human GSDME aa 1-496.

Uniprot id :

O60443>SwissProt: O60443 Human;SwissProt: Q9Z2D3 Mouse

Host :

Rabbit

Clone number :

PSH10-84

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Cytoplasm, cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IP: 1-2μg/sample

Advanced Validation :

Cell treatment (CT),Relative expression (RE)

more info or order :

HUABIO product webpage