Human CSPG4 | HUABIO HA723246 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human CSPG4

catalog :

HA723246

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-79

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723244) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723246, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Sandwich ELISA analysis of Human CSPG4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723245) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSPG4 protein (HA211047) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723246, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 3 :

Interpolated concentrations of native CSPG4 in A-375, SK-MEL-28, SK-BR-3 and MCF7 cell culture supernatant. The concentrations of CSPG4 were measured in duplicates, interpolated from the CSPG4 standard curve and corrected for sample dilution. Undiluted samples are A-375 cell culture supernatant 25%, SK-MEL-28 cell culture supernatant 100%, SK-BR-3 cell culture supernatant 100% and MCF7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSPG4 concentration was determined to be 164.9 ng/ml in A-375 cell culture supernatant, 17.2 ng/ml in SK-MEL-28 cell culture supernatant and undetectable in SK-BR-3 and MCF7 cell culture supernatant.

product information

SKU :

HA723246

Target name :

Human CSPG4

Species reactivity :

Human

Applications :

ELISA(Det)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human CSPG4 aa 1356-2224 (HA211047).

Uniprot id :

Q6UVK1>SwissProt: Q6UVK1 Human

Host :

Rabbit

Clone number :

PSH10-79

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human CSPG4 protein (HA211047).

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Det): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-77] to Human CSPG4 antibody (Capture) (HA723244) or Rabbit monoclonal [PSH10-78] to Human CSPG4 antibody (Capture) (HA723245) and Recombinant Human CSPG4 protein (HA211047) as the standard. The reference range value is 156-20,000 pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723246_4.png

Pic legend4 :

Interpolated concentrations of spiked CSPG4 in human cell culture media samples. The concentrations of CSPG4 were measured in duplicates, interpolated from the CSPG4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

more info or order :

HUABIO product webpage