Human MICB | HUABIO HA723238 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human MICB

catalog :

HA723238

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-73

reactivity :

human

application :

ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Sandwich ELISA analysis of Human MICB matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723238) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MICB protein (HA210907) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723239, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

image 2 :

Interpolated concentrations of native MICB in T-47D and PC-3 extract samples based on a 1,000 µg/ml extract load. The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curve and corrected for sample dilution. Undiluted samples are T-47D extract 100% and PC-3 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 5,368 pg/ml in T-47D extract and undetectable in PC-3 extract.

image 3 :

Interpolated concentrations of native MICB in MCF-7 and PC-3 cell culture supernatant. The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curve and corrected for sample dilution. Undiluted samples are MCF-7 cell culture supernatant 100% and PC-3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 334.07 pg/ml in MCF-7 cell culture supernatant and undetectable in PC-3 cell culture supernatant.

product information

SKU :

HA723238

Target name :

Human MICB

Species reactivity :

Human

Applications :

ELISA(Cap)

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human MICB aa 23-309 (HA210907).

Uniprot id :

Q29980>SwissProt: Q29980 Human

Host :

Rabbit

Clone number :

PSH10-73

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Recombinant Human MICB protein (HA210907).

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA(Cap): Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-74] to Human MICB antibody (Detector) (HA723239) and Recombinant Human MICB protein (HA210907) as the standard. The reference range value is 39.1-5,000 pg/ml.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723238_4.png

Pic legend4 :

Interpolated concentrations of spiked MICB in human cell culture media samples. The concentrations of MICB were interpolated from the MICB standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.

more info or order :

HUABIO product webpage