BLBP | HUABIO HA723232 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BLBP

catalog :

HA723232

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-68

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Application: IF-tissue Species: Mouse Site: Cerebral cortex (P7) Sample: Paraffin-embedded section Antibody concentration: 1:1,000

image 3 :

Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1:1,000

product information

SKU :

HA723232

Target name :

BLBP

Species reactivity :

Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue

Conjugate :

Non-conjugated

Uniprot id :

P51880>SwissProt: P51880 Mouse;SwissProt: P55051 Rat

Host :

Rabbit

Clone number :

PSH10-68

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse P7 brain tissue, mouse cerebellum tissue, Mouse cerebellum (P0) tissue lysate, Mouse cerebellum (P7) tissue lysate, Rat cerebellum tissue lysate.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:2,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723232_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse P7 brain tissue with Rabbit anti-BLBP antibody (HA723232) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723232) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723232_5.jpg

Pic legend5 :

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723232_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-BLBP antibody (HA723232) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723232) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723232_7.jpg

Pic legend7 :

Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (HA723232) at 1/5,000 dilution. Lane 1: Mouse cerebellum (P0) tissue lysate Lane 2: Mouse cerebellum (P7) tissue lysate Lane 3: Mouse kidney tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723232) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA723232_8.jpg

Pic legend8 :

Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (HA723232) at 1/5,000 dilution. Lane 1: Rat cerebellum tissue lysate Lane 2: Rat kidney tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723232) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage