CD48 | HUABIO HA723230 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD48

catalog :

HA723230

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-65

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA723230) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: Ramos cell lysate Lane 4: THP-1 cell lysate (negative) Lane 5: Daudi cell lysate Lane 6: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 40-50 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723230) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of HeLa (left, negative) and Daudi (right, positive) cells labeling CD48. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723230, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

Flow cytometric analysis of THP-1 (left, negative) and Daudi (right, positive) cells labeling CD48. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723230, 1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA723230

Target name :

CD48

Species reactivity :

Human

Applications :

WB,FC,IP,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CD48 aa 27-220.

Uniprot id :

P09326>SwissProt: P09326 Human

Host :

Rabbit

Clone number :

PSH10-65

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Raji cell lysate, Ramos cell lysate, Daudi cell lysate, Jurkat cell lysate, Daudi, human colon tissue, human tonsil tissue, human lung adenocarcinoma tissue.

Molecular wt :

Predicted band size: 28 kDa

Subcellular location :

Cell membrane, Membrane raft, Secreted.

Concentration :

0.2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;FC: 1:2,000 ;IP: 1-2μg/sample ;IHC-P: 1:600-1:2,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA723230_4.jpg

Pic legend4 :

CD48 was immunoprecipitated from 0.2 mg Daudi cell lysate with HA723230 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723230 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Daudi cell lysate (input) Lane 2: HA723230 IP in Daudi cell lysate Lane 3: Rabbit IgG instead of HA723230 in Daudi cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 4 seconds; ECL: K1801

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA723230_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD48 antibody (HA723230) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723230) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA723230_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD48 antibody (HA723230) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723230) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA723230_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-CD48 antibody (HA723230) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723230) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage